Day 3

DAY 3

The third day of lab we learned about the characteristic features of bacterial growth in a culture by looking at bacteria in our Agar plates.

For bacteria growing on Agar plates there are different options in which the colony can be classified by

·         In a whole colony  (form) you can have: puntiform, circular, rhizoid, spindle, irregular, filamentous, or concentric.

·         The different colony pigmentations you can have included: colorless white, purple, yellow, cream, tan, blue-green, rose or red.

 ·         The appearance of colony surfaces include: opaque, transparent, translucent, rough or dull, smooth of glistening

·         The colony margin (edge) includes entire, undulate, lobate, filamentous, and irregular (erose)

·         There are different elevations of the colony, which include: flat, raised, convex, pulvinate, umbonate, hily, ingrowing, or crateriform.

For Bacteria growing on an Agar slant there are different options for what the colony can be classified by. It is either arborescent (branched), beaded, echinulate (pointed), filiform (even), rhizoid (rootlike), or spreading.

For bacteria growing in a broth medium it spans from no growth to turbid. If there is growth it is either sediment, pellicle, ring, or flocculent.

For our Agar plant we found the flowing observations.

·         whole colony form: circular

·         colony pigment: cream-colored pigment

·         appearance of colony surface: opaque

·         colony margin (edge): entire

·         Elevation of colony: raised

For our agar slant we found that it was filiform (even)

For bacteria growth in our broth medium we found that it was between no growth and turbid. Specifically we found that our bacteria was flocculent.

We then made another Agar plate, and swabbed Graces mouth with a swab and spread it onto the plate and placed it in the incubator to grow. We will look at the results for this environmental sample next class.

We also made a spread plate with bacteria and on it we wrote JMJ with a virus called bacteriophage, which would kill the bacteria. We found that it did kill the bacteria and carved JMJ into the spread plate.